In this proof of principle experiment, we demonstrate that MALDI-MSI combined with classical LCM LC-MS/MS has the potential to spatially resolve metabolic signatures in human brain at cellular resolution. Abstract & Talk given - The Oxford Metabolic Health Symposium 2020, 30th June – 3rd July 2020.
LC-MS based mass spectrometry imaging of proteins has the potential to provide novel insights into the proteome of tissues. Abstract - Presented at the Oxford Metabolic Health Symposium 2020, 30th June – 3rd July 2020.
Here we apply a sensitive, spatially resolved workflow for the proteomic analysis of a tumour to identify proteins that display spatial expression patterns within the tissue in an unsupervised manner. Abstract and Poster - ASMS Conference on Mass Spectrometry and Allied Topics, May 31st - June 4th 2020.
Linking tissue microstructure with diffusion MRI signals throughout the brain Abstract and Poster - Whistler Scientific Workshop on Brain Functional Organization, Connectivity, and Behavior, March 1st - March 4th 2020.
Presented at 121st Meeting of the British Neuropathological Society, Developmental Neuropathology Symposium, 4th – 6th March 2020. Published Poster - Journal of Neuropathology and Applied Neurobiology.
The detection of specific TDP-43 peptides able to quantify the pathological fragments of TDP-43 in post mortem ALS brain tissue, offers the opportunity to develop an in vivo assay to measure pathological TDP-43. This would have major diagnostic, stratification and pharmacodynamic biomarker potential. The finding of CTF in cases labelled as AD may reflect the recent identification of a sub-group labelled Limbic-predominant Agerelated TDP-43 Encephalopathy. Published Abstract (Journal of Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration, volume 20, S1, November 2019) and Poster - 30th International Symposium on ALS/MND, December 4th - 6th, 2019.
Diffusion MRI (dMRI) has great potential for studying the complexity of white matter fibre architecture non-invasively. However, because dMRI is an indirect measure of this microstructure, we require validation datasets for two main purposes. (i) to relate dMRI to microscopy data that directly measures the microstructure of interest; and (ii) to relate high-quality dMRI data to more conventional data quality. We present the NAMETBC dataset which addresses both of these goals. Article and Poster - International Society for Magnetic Resonance in Medicine (ISMRM) 27th Annual Meeting & Exhibition, May 11th - 16th, 2019.
Here we describe a method to increase proteomic throughput by 10-times. The 10x increase in throughput increases possibilities for investigation into the spatial distribution of proteins throughout a tissue. We will next apply this methodology in order to determine spatial proteomic profiles of distinct histological features within a brain tumour. Abstract and Poster - 18th Human Proteome Organisation World Congress (HUPO2019), Australia, September 15th - 19th 2019.
The coupling of laser capture microdissection and proteomics is a powerful technique capable of generating reproducible, quantitative proteomes of sufficient depth from a single type of cell isolated from a mixed population, which has the potential to yield unbiased information about mechanisms of cell fate specification and degeneration in the human brain. Abstract and Poster - Human Proteome Organisation World Congress (HUPO2018), September 30th - October 03th 2018.